Abstract: Thermoacoustic refrigeration technology, known for its high reliability and significant environmental friendliness, relies heavily on the performance of the regenerator. To efficiently pump heat at different temperatures in the regenerator, a four-stage looped thermoacoustic refrigerator utilizing a multi-layered mesh regenerator is proposed and numerically simulated. The computational results indicate that the design of a multi-layered regenerator is conducive to ensuring efficient heat exchange while reducing viscous dissipation, thereby enhancing refrigeration capacity and efficiency. Among the investigated configurations, the system performance is most optimal with a three-layer mesh regenerator. Under the conditions of using 6 MPa high-pressure helium gas, a hot end temperature of 923 K, and a room temperature end of 303 K, the refrigeration system achieved a cooling capacity of 109 W at 77 K, surpassing the conditions with one, two and four-layer regenerators.