Abstract: After the lunar sample sealing device comes back to the ground，the device must be unsealed; sample must be transferred，stored，prepared and analyzed.If lunar sample is exposed to the air during these procedures，lunar sample reacts with the atmosphere.For lunar sample protection and anti-pollution，this paper introduces the anti-pollution method during the whole process，presents the design idea about lunar sample unsealing and separating device and calculates the atmospheric index in anti-pollution device.