Abstract: Data transmission software for safety monitoring system of bulkhead is a special test software to improve timeliness，accuracy，reliability of measurement.This paper mainly introduces functional requirements and structure design of data transmission software，data acquisition module design of mass spectrometer，network transmission function design，and analysis of functional test results.By using modular design，OPC for mass spectrometer，TCP/IP for network and SQL database，data transmission software implemented automatic data acquisition，storage and transmission of bulkhead residual gas concentration measurement in the process of cryogenic liquid rocket launch，and simplified operation process of launch site.