Abstract: Manufacturing of microfluidic chip based on glass has many problems like complex craft, long-term processing cycle and high cost. This experiment improves these problems with glass and PDMS as raw materials, makes wellstructured micro-channels on the glass and PDMS in the way of wet corrosion, and obtains two chips by irreversible sealing. In the research, glass/PDMS chip(channel in the glass) and glass/PDMS chip(channel in PDMS) are made and studied their volt-ampere characteristics. It is concluded that the highest voltage of the chip linear volt-ampere characteristics are 1 990 V, 1 050 V, respectively, in accordance with the field strength of 499 V·cm^-1 and 263 V·cm^-1. The electro-osmosis properties of microfluidic chips are analyzed by the method of current monitoring, whereby it is found that the electric seepage velocity grows with the increase of buffer solution pH and adding surfactant SDS can dramatically raise the velocity and stability of the electro-osmotic.