Abstract: In order to study the liquefaction and layering of hydrogen isotope,a large number of off-line experiments must be carried out to obtain the parameters. In this paper,the adaptive vibration cooling/damping/heater transfer technology based on G-M mechanical refrigeration is adopted,and the sample holder for off-line experiment is designed and developed. The process parameters of preparing low-temperature experimental samples are obtained,which provides technical support for the preparation of samples for the cryogenic physics. The results show that the temperature condition of offline sample holder is the same as formal cryogenic physical experiment,which the minimum temperature of the target frame is 8.4K and the vibration amplitude of the target is less than ±400 nm.